Expression of matrix metalloproteinase related gelatinases in rats′optical nerves after extrusion wound
- VernacularTitle:大鼠视神经挤压伤后基质金属蛋白酶-2和-9的表达及意义
- Author:
Shuihua ZHANG
;
Changguan WANG
;
Zhizhong MA
- Publication Type:Journal Article
- Keywords:
Matrix metalloproteinases;
Optical nerve injuries/enzymology;
Disease models, animal
- From:
Chinese Journal of Ocular Fundus Diseases
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of matrix metalloproteinase (MMP)-2 and MMP-9 in rats′ optical nerves after extrusion wound. Methods We set up the model of rats with extrusion wound of the optical nerves, detected activity changes of MMP-2 and MMP-9 in the optical nerves by gelatin zymography, identified the attribute by Western blotting, and verified the expression of mRNA of MMP-2 and MMP-9 by reverse transcriptase-polymerase chain reaction (RT-PCR). Results MMP-2 existed in normal optial nerves and optical nerves with extrusion wound, while MMP-9 was only detected in the latter. The expression of MMP-9 was the highest 1 day after the extrusion wound, while that of MMP-2 was the highest 7 days after the extrusion wound. Conclusions MMP-2 and MMP-9 may participate in the pathological recovery process of optical nerves after extrusion wound. The glial cells in the optical nerves may be one of the sources of MMP-2 and MMP-9.
