Effect of oxygen inhalation on the retinae of newborn rats and its mechanism
- VernacularTitle:吸氧对新生鼠视网膜的影响及其机制
- Author:
Hongbin XU
;
Xiaolong CHEN
- Publication Type:Journal Article
- Keywords:
Retinopathy of prematurity;
Superoxide dismutase;
Malondialdehyde;
Disease models, animal
- From:
Chinese Journal of Ocular Fundus Diseases
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of oxygen inhalation on the retinae of newborn rats and its mechanism. Methods We mimicked the retinopathy of prematurity(ROP) by putting the newborn rats in high concentrated oxygen. One-day-old rats were put into the oxygen box with the oxygen concentration of 80% for continuous 7 days; then in air condition for 7 days. The arterial blood oxygen pressure, retinal superoxide dismutase (SOD), and malondialdehyde (MDA) of the rats (1,2,4,7,8,9,11,14 days old) were examined. The diameter of retinal vessels′ main branch and the coverage rate of peripheral vessels were measured in 7- and 14-day-old rats by ink perfusion. The retinal neovascularization of rats (8,9,11,14 days old) were observed by HE staining. The rats of the same age fed in air condition were in the control group. Results The differential pressures of blood oxygen of rats (1,2,4,7 days old) in study group were significantly higher than those in the control group (P0.05). The contents of SOD of the retinae in the rats (1,2,4,7,8 days old) were significantly lower than those in the control group(P
