Inhibitory effect of hydrogen sulfide (H_2S) on cultured rat aortic smooth muscle cells
- VernacularTitle:硫化氢对培养的大鼠主动脉平滑肌细胞增殖的抑制作用
- Author:
Xiaobo CHEN
;
Junbao DU
;
Bin GENG
;
Hongfeng JIANG
;
Chaosh TANG
- Publication Type:Journal Article
- Keywords:
Hydrogen sulfide;
Endothelin-1;
Mitogen-activated protein kinases;
Muscle, smooth, vascular;
Rats
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effects of hydrogen sulfide (H_2S) on proliferation of vascular smooth muscle cells (VSMC) stimulated by endothelin (ET-1, 10 -7 mol/L ) and mitrogen-activated protein kinase (MAPK) activity in VSMCs. METHODS: Cultured VSMCs were divided into six groups: (1) control group, (2) serum group, (3) endothelin group, (4) NaHS groups, (5) serum+NaHS group, and (6) endothelin+NaHS group. VSMC proliferation was measured by [ 3H]-TdR incorporation and MAPK activity in VSMC was determined by radioactivity assay. RESULTS: ET-1 increased VSMC [ 3H]-TdR incorporation by 2.39 times ( P
