Expression and functional analysis of novel gene AngRem104 associated with glomerular sclerosis
- VernacularTitle:人肾小球系膜细胞增生硬化相关新基因AngRem104的表达和功能研究
- Author:
Xiubin LIANG
;
Hong ZHANG
;
Haiyan WANG
- Publication Type:Journal Article
- Keywords:
Angiotensin Ⅱ;
Fibronectins;
Gene function;
Genes, AngRem;
Glomerulosclerosis, focal
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the expression and function of novel gene AngRem104. METHODS: Northern blot was performed to detect the distribution of AngRem104 in human multiple normal tissues as well as the effect of AngⅡ and AT1R antagonist (losartan) on AngRem104 expression. The sense and antisense eukaryotic expression vectors of AngRem104 were constructed and transfected into human mesangial cells. RT-PCR was used to detect the expression of FN when AngRem104 was over-expressed. Primary sequence and motif analysis of AngRem104 protein were performed by on-line ExPasy predictive tools. RESULTS: AngRem104 was predicted to localize at the cellular nucleus. It was widely expressed in human heart, placenta, liver, muscle, kidney and pancreas. Moreover, the up-regulated expression of AngRem104 induced by AngⅡ was inhibited by losartan in a dose-dependent manner. CONCLUSION: AngRem104 is a novel nuclear protein related to the expression of fibronectin and could be up-regulated by AngⅡ in human MC.
