Analysis of gene expression profile of multidrug resistant MCF/DOX cell line after benflumetol derivative LY980503 treatment
- VernacularTitle:本芴醇衍生物LY980503对人乳腺癌多药耐药细胞株MCF/DOX基因表达谱的影响
- Author:
Feng HUANG
;
Yongling WAN
;
Dalong WU
;
Huanzhang L
;
Junhu GUO
- Publication Type:Journal Article
- Keywords:
Oligonucleotide array sequence analysis;
Breast neoplasms;
Gene expression;
Drug resistance, multiple
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of LY980503(a benflumetol derivative)on multidrug resistance of tumor cell line using DNA microarray. METHODS: Total RNA was extracted from multidrug resistant MCF/DOX cell line. cDNA microarray containing 320 cDNAs was used to detect the gene expression profile. RESULTS: 9 down-regulated genes and 1 up-regulated gene were identified after multidrug resistant MCF/DOX cells were treated with LY980503. CONCLUSION: LY980503 can effectively reverse the resistance of MCF/DOX to DOX in vitro by adjusting the expression of multi-genes.
