Overexpression of neurokinin-1 receptor is associated with mucosal damage in acute necrotizing pancreatitis
- VernacularTitle:急性坏死性胰腺炎肠粘膜损害与NK-IR的过度表达相关
- Author:
Xin SHI
;
Nairong GAO
;
Yongjiu YANG
;
Mingdong HUO
;
Haolin HU
;
Qingmin GUO
- Publication Type:Journal Article
- Keywords:
Substance P;
Receptor, neurokinin-1;
Receptor, neurokinin-2;
Pancreatitis
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the expression and the role of neurokinin-1 receptor(NK-1R) and neurokinin-2 receptor (NK-2R) in distal ileum during acute necrotizing pancreatitis(ANP).METHODS: 130 adult Sprague-Dawley rats were divided into 2 groups: the rats in ANP group( n=80 ) were induced by the retrograde intraductal infusion of 5% sodium taurocholate. The rats in sham operation control group ( n=50 ) received laparotomy only. Reverse transcription polymerase chain reaction (RT-PCR) was used to determine the mRNA expression of NK-1R and NK-2R, Western blot was used to investigate expression level of NK-1R.RESULTS: NK-1R and NK-2R mRNA levels were enhanced in the distal ileum of ANP rats compared with controls. Western blot revealed stronger NK-1R immunoreactivity exited in intestinal mucosa in ANP rats. The overexpression of NK-1R was associated with mucosal pathological score( r=0.77,P
