Transfection and expression of murine interleukin-12 gene in B16F10 melanoma cells
- VernacularTitle:小鼠白细胞介素12基因转染及其在黑色素瘤细胞B16F10中的表达
- Author:
Jun ZHAO
;
Fenyong SUN
;
Chen LI
;
Yanling YAN
;
Yife ZHANG
- Publication Type:Journal Article
- Keywords:
Interleukin-12;
Gene transfection;
Gene expression;
Melanoma
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the expression of murine interleukin-12(mIL-12) gene in B16F10 melanoma cells. METHODS: mIL-12 gene was inserted into pcDNA3.1 eukaryotic expression vector by DNA recombination and then transfected into B16F10 melanoma cells by electroporation. The integration and expression of mIL-12 gene in B16F10 melanoma cells were detected by PCR,RT-PCR and Western blot after positive cell clones had been screened. RESULTS: mIL-12 gene was confirmed to have been transfected and expressed in B16F10 cells on three levels of DNA, mRNA and protein. CONCLUSION: mIL-12 gene can successfully be transfected and expressed in B16F10 melanoma cells in vitro , which lays a foundation for the further investigation of mIL-12 gene tumor vaccine.
