Effects of selective iNOS inhibitor 1400W on expired nitric oxide and plasma nitrate concentration in a porcine model of endotoxemia
- VernacularTitle:1400W对内毒素血症猪呼出气一氧化氮及血浆硝酸盐水平的影响
- Author:
Yan YANG
;
Shanglong YAO
;
Shiying YUAN
- Publication Type:Journal Article
- Keywords:
Endotoxemia;
Nitric-oxide synthase;
Nitric oxide;
Enzyme inhibitors;
1400W
- From:
Chinese Journal of Anesthesiology
1996;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of selective inducible nitric oxide synthase (iNOS) inhibitor 1400W on nitric oxide (NO) exhalation and plasma nitrate concentration during endotoxemia in pigs. Methods Fourteen pigs of both sexes aged 12-16 weeks, weighing 40-60 kg were randomly divided into two groups : ( Ⅰ ) endotoxemia group ( n = 6) and ( Ⅱ ) 1400W group ( n = 8). The animals were anesthetized with pentobarbital and ketamine, intubated and mechanically ventilated. PaC02 was maintained at 35-45 mm Hg. Femoral artery and portal vein were cannulated for BP monitoring and blood sampling. E coli (LPS 20 mg L-1 ) was continuously infused iv at 4 ml h-1 for 24 h in both groups. In 1400W group, at 12 h of LPS infusion, continuous iv infusion of 1400W was started at 0.5 mg kg-1 h-1 . Blood samples were taken from artery and portal vein before LPS infusion (T0) and at 12 h (T, ) and 24 h (T2) of LPS infusion for determination of plasma nitrate concentration. Expired NO concentration was measured using NOA 280 NO analyzer. Results 1400W decreased LPS-induced increase in expired NO (P 0.05). Conclusion The selective iNOS inhibitor 1400W can inhibit iNOS activity and lead to decreased NO production.
