The effects of different pneumoperitoneum on in vitro tumor cells growth
- VernacularTitle:不同气腹介质对肿瘤细胞体外生长的影响
- Author:
Xianying MENG
;
Guangyi WANG
;
Shufen XU
;
Jianhua GU
- Publication Type:Journal Article
- Keywords:
Pneumoperitoneum;
Carbon dioxide;
Helium;
Air;
Neoplasms, malignant
- From:
Chinese Journal of General Surgery
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThis study was to evaluate the effects of different pneumoperitoneum used in laparoscopy on in vitro tumor cells growth. Methods 3H-TdR incorporation was used to compare the DNA synthesis (CPM value) of tumor cells after exposure to simulated laparoscopic environments, composed of carbon dioxide, helium and room air, respectively. Results At 24 hours after pneumoperitoneum, compared with group of helium, air and control, CPM increased in group of carbon dioxide (F=47.576 5,P0.05). At 48,72 and 96 hours, pneumoperitoneum, compared with helium group, air group and control group, CPM increased significantly in carbon dioxide group (F=116.183,1082.92 and 5116.75, P0.05). Conclusion Carbon dioxide promotes tumor cells growth, while helium restrains the growth of tumor cells. [
