Study on mast cells of intestinal mucosa in irritable bowel syndrome patients under light and electron microscope
- VernacularTitle:肠易激综合征患者肠黏膜肥大细胞的光镜和电镜观察
- Author:
Wenzhu DONG
;
Zhaoshen LI
;
Duowu ZOU
- Publication Type:Journal Article
- Keywords:
Irritable bowel syndrome;
Mast cell;
Substance P;
Substance P receptor
- From:
Chinese Journal of Digestive Endoscopy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether there are changes of the mast cells (MCs) and substance P ( SP) -ergic nerve terminals under light and electron microscopy, and to elucidate their possible roles in the pathogenesis of irritable bowel syndrome (IBS). Methods In 19 normal controls, 22 diarrhea predominant IBS ( D-IBS) and 20 constipation predominant IBS (C-IBS) , biopsies were taken from the terminal ileum, ileocecal junction, ascending colon and sigmoid colon. The MCs and the substance P (SP)-ergic nerve terminals, substance P receptor ( SPR) were stained by histochemical and immunohisto-chemical method respectively, and color image analyzer investigated the results qualitatively and quantitatively. The structure relation between the MCs and SP-ergic terminals was studied through an ultra-microscopy using in situs embedding technique and a light microscopic study in serial sections. Results The number of MCs in the terminal ileum, ileocecal junction, and ascending colon was significantly elevated in IBS (P
