Study on loss of heterozygosity of SMAD2 in primary gastric carcinoma
- VernacularTitle:原发性胃癌SMAD2杂合性丢失的研究
- Author:
Yaqing ZHU
;
Haoran YIN
;
Zhenggang ZHU
;
Bingya LIU
;
Yi ZHANG
;
Xuehua CHEN
;
Yingyan YU
;
Yanzhen LIN
- Publication Type:Journal Article
- Keywords:
STOMACH NEOPLASMS/genet;
SMAD2;
HETEROZYGOSITY LOSS;
STOMACH NEOPLASMS/pathol
- From:
Chinese Journal of General Surgery
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of loss of heterozygosity (LOH) of SMAD2 and its relationship with clinicopathological parameters of primary gastric carcinoma. Methods Fifty cases of primary gastric carcinoma were monitored by polymerase chain reaction -single strand conformation polymorphism (PCR- SSCP) and silver staining to detect SMAD2 LOH. Results The incidence of LOH was 40.0%(22/45) at D18S450 and 33.3%(16/48)at D18S460 . LOH occurred in SMAD2 was 55.1%(27/49).The rate of SMAD2 LOH was 72.0% (18/25) in primary gastric carcinoma with lymph node metastasis , which was significantly higher than that in without lymph node metastasis (P
