Mechanism of pulmonary arterial smooth muscle cell apoptosis induced by nitric oxide
- VernacularTitle:一氧化氮诱导大鼠肺动脉平滑肌细胞凋亡机制研究
- Author:
Hong TIAN
;
Junbao DU
;
Jin FAN
;
Weihong ZHAO
;
Chaosh TANG
- Publication Type:Journal Article
- Keywords:
Nitric oxide;
Pulmonary artery;
Muscle, smooth, vascular;
Apoptosis;
Rats
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the mechanism of apoptosis in pulmonary arterial vascular smooth muscle cells (PASMCs) induced by nitric oxide (NO). METHODS: Wistar rat PASMCs were isolated from explants from the intrapulmonary, incubated with NO donor, sodium nitroprussid (SNP) under 12 or 24 hour normoxic and hypoxic conditions. The cell cycle progression and sub-G 1 of PASMC were analyzed via flow cytometric staining of propidium iodide, and the expression of nuclear transcription factor NF-?B and pro-apoptotic factors caspase-3 were detected using immunochemistry staining. Meanwhile gel electrophoresis of extracted DNA by PASMC was performed. RESULTS: SNP induced PASMC apoptosis in a dose-dependent manner ( P
