The influence of peroxisome proliferator-activated receptors on transcription of plasminogen activator inhibitor-1 in endothelial cells
- VernacularTitle:过氧化体增殖物激活型受体对血管内皮细胞PAI-1转录的调节
- Author:
Xiaohui HU
;
Ping YE
;
Yongxue LIU
;
Haijin SONG
- Publication Type:Journal Article
- Keywords:
Endothelium, vascular;
Peroxisome proliferator -activated receptors;
Plasminogen inactivators
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression of peroxisome proliferator-activated receptors (PPARs) in human endothelial cells, and their effects on plasminogen activator inhibitor-1 transcription. METHODS: The expression of three types of PPARs in mRNA level were detected in human umbilical vein endothelial cells(HUVECs) by using RT-PCR. Cultured endothelial cells line-ECV304 were transfected with PAI-1 promoter controlling CAT reporter gene and co-transfected with varying doses (250, 500, 1 000 ng) of expression vectors PPAR? or PPAR?.The transcripton activity of PAI-1 promoter were detected with ELISA. RESULTS: There were all three types of PPARs mRNA expression in HUVECs, while the expression of PPAR? was less than that of PPAR?( P
