Determination of the Content of Puerarin in Compound Rhizoma Coptidis Graininess by HPLC
- VernacularTitle:高效液相色谱法测定复方黄连颗粒中葛根素的含量
- Author:
Liqin TANG
;
Sheng LIU
;
Liming CHEN
;
Chu LI
- Publication Type:Journal Article
- Keywords:
HPLC;
compound Rhizoma Coptidis graininess;
puerarin;
content
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for determination of the content of puerarin in compound Rhizoma Coptidis graininess METHODS:HPLC method was used The samples were separated by C18 (4 6mm?250mm,5?m) column with the mobile phase of methanol-water(30∶70) The detection wavelength was set at 250nm Flow rate was 1 0ml/min Column temperature was 30℃ RESULTS:The linear ranges of puerarin was 0 312?g～1 560?g The average recovery was 99 2%,RSD=2 08%(n=5) CONCLUSION:The method is sensitive,rapid and accurate and suitable for the determination of puerarin in compound Rhizoma Coptidis graininess
