Determination of the Content of Berberine Hydrochloride in Weibining Tablets by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定胃必宁片中盐酸小檗碱的含量
- Author:
Yuan YAN
;
Dan GUO
;
Zhiliang CHEN
- Publication Type:Journal Article
- Keywords:
Weibining tablet;
berberine hydrochloride;
RP-HPLC;
content
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the content of berberine hydrochloride in Weibining tablets METHODS:RP-HPLC was used with Nova-Pak C18 column as stationary phase The mobile phase was 0 033mol/L postassium dihydrogen phosphate-acetonitrile(60∶40) The UV detection wavelength was 265nm RESULTS:There was a good liner relationship within the concentration range of 0 32～1 6?g/ml of berberine hydrochloride(r=0 9 999) The average recovery was 99 07%(n=5,RSD=0 85%) CONCLUSION:This method is simple,fast and accurate
