Changes of Caspase 3mRNA Expression After Rat Focal Cerebral Ischemia/Reperfusion Injury Treated by Mild Hypothermia
- VernacularTitle:亚低温处理大鼠脑缺血再灌注损伤后Caspase-3mRNA的表达变化
- Author:
Guoxiang WU
;
Shanping MAO
;
Xinghan HUANG
- Publication Type:Journal Article
- Keywords:
Mild hypothermia;
Cerebral ischemia/reperfusion injury;
Caspase 3mRAN;
Apoptosis
- From:
Journal of Chinese Physician
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the changes of Caspase 3mRNA expression and the injuried nervous cell apoptosis after rat focal cerebral ischemia/reperfusion injury who was then treated by mild hypothermia.Methods The middle cerebral arteries(MCA) of SD rats were occluded for 2 hours,and reperfused for 12 hours.Using FCM and semiquantitative RT-PCR technique,the DNA fragmentation rate and Caspase 3mRNA expression level were detected in the shamoperation group,the control group and the mild hypothermia group,respectively.Results The DNA fragmentaation rate and Caspase 3mRNA expression level in the sham operation group and the mild hypothermia group were obviously lower than those in the control group.Conclusions The significantly decreased level of Caspase-3mRNA expression may be related to the obviously decreased nervous cell apoptosis after rat cerebrall ischemia/reperfusion injury treated by the mild hypothemia.
