Expression of Haptoglobin in Normal Human Epidermal Cells and HaCaT Cells
- VernacularTitle:结合珠蛋白在正常人表皮细胞及HaCaT细胞中的表达
- Author:
He WANG
;
Xinghua GAO
;
Yakun WANG
;
Ping LI
;
Chundi HE
;
Yuming ZHAO
;
Yong XIE
;
Hongduo CHEN
- Publication Type:Journal Article
- Keywords:
Globin;
Keratinocytes;
Langerhans cells;
HaCaT cells
- From:
Chinese Journal of Dermatology
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of haptoglobin(Hp)mRNA and protein in nor-mal human epidermal cells and human keratinocyte cell line-HaCaT cells.Methods In situ hybridization and RT-PCR were used to detect the expression of Hp mRNA in normal human epidermal cells and HaCaT cells.Immunohistochemistry was used to detect the expression of Hp on normal human epidermal cells.Im-munohistochemistry and Western blot were used to detect Hp expression on HaCaT cells.Results There was Hp mRNA expression in normal human keratinocytes and HaCaT cells.There was no Hp mRNA expres-sion in normal human epidermal Langerhans cells.There were some Hp positive dendritic cells in normal human epidermis.There was no obvious Hp protein staining in the HaCaT cells by immunohistochemistry.There was Hp protein band from HaCaT cells by Western blot.Conclusions There is Hp mRNA expression in normal human keratinocytes and HaCaT cells which suggests that normal human keratinocytes and HaCaT cells have the ability to synthesize Hp protein.Normal human epidermal Langerhans cells have no ability to synthesize Hp protein.There is small amount of Hp protein in HaCaT cells.
