Oxidized low density lipoprotein increases the expression of matrix metalloproteinase-2 in vascular endothelial cells
- VernacularTitle:氧化低密度脂蛋白促进血管内皮细胞表达基质金属蛋白酶-2
- Author:
Feng HAO
;
Jinde YU
- Publication Type:Journal Article
- Keywords:
Matrix metalloproteinases;
Lipoproteins, LDL;
Endothelium;
Atherosclerosis
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of oxidized low density lipoprotein (OX-LDL) on expression of matrix metalloproteinase-2 (MMP-2) in human umbilical vein endothelial cells (HUVEC). METHODS: The MMP-2 mRNA was determined by reverse transcriptase-polymerase chain reaction (RT-PCR), MMP-2 protein was measured by western blotting and MMP-2 activity in conditioned medium was observed by gelatin zymography. RESULTS: In comparison with the control, there was no difference in the expression of MMP-2 between control and 25 mg/L LDL, whereas 25 mg/L OX-LDL increased MMP-2 activity in HUVEC ( P
