Effects of homocysteine on the expression of macrophage inflammatory protein-1? in cultured human umbilical vein endothelial cells
- VernacularTitle:同型半胱氨酸对培养的人脐静脉内皮细胞巨噬细胞炎性蛋白1?表达的影响
- Author:
Shuxiu WANG
;
Feiyan ZOU
;
Zhongduan DENG
;
Zhiling QU
;
Juan NI
- Publication Type:Journal Article
- Keywords:
Homocysteine;
Endothelium, vascular;
Macrophage inflammatory protein;
Atherosclerosis
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of homocysteine (HCY) on the induction of macrophage inflammatory protein-1? (MIP-1?) expression in cultured human umbilical vein endothelial cells (HUVECs). METHODS: After exposure of the cultured HUVECs to HCY at increasing concentrations (0.1, 0.5 and 1 mmol/L) for 8 h, the MIP-1? mRNA expression was determined by in situ hybridization using a MIP-1? cDNA probe, and the MIP-1? protein expression was measured by cell enzyme-linked immunosorbent assay (ELISA) using a goat anti-human MIP-1? monoclonal antibody. RESULTS: The in situ hybridization showed that cultured HUVECs were able to express MIP-1? mRNA at a low level that was purplish blue granules in cytoplasm. After exposure to HCY at the concentrations mentioned above, the expression of MIP-1? mRNA was significantly increased in a dose-dependent manner. Analysis of variance showed that there was significant difference between groups ( F= 606.38, P
