Preliminary study on detecting drug-resistance mycobacterium tuberculosis by using DNA microarray hybridization
- VernacularTitle:利用基因芯片技术快速检测结核杆菌耐药性的初步研究
- Author:
Wanjiang ZHANG
;
Lang BAO
;
Xiaoying WAN
;
Huidong ZHANG
;
Yongen XIE
;
Wei CHEN
;
Xianghua YU
- Publication Type:Journal Article
- Keywords:
Oligonucleotide array sequence analysis;
Mycobacterium, tuberculosis;
Drug tolerance
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To detect the drug-resistance mycobacterium tuberculosis by using DNA microarray hybridization.METHODS: DNA microarray for detecting drug-resistance of mycobacterium tuberculosis was prepared; Clinical isolated strains were cultivated and their drug-resistance sensitivity was detected. The genome DNA of mycobacterium tuberculosis was prepared and the drug-resistance genes of the mycobacterium tuberculosis were amplified by PCR. Then the gene chip was hybridized, washed, detected and analyzed. RESULTS: Results of cultivating mycobacterium tuberculosis and detecting the drug-resistance sensitivity: one strain was drug-sensitive; four strains were multi-drug-resistant; The detecting results of the drug-resistance was consistent with the results of diagnosis therapy of the 5 clinical patients. The detecting results of gene chip confirmed the above facts. CONCLUSION: Detecting drug-resistance mycobacterium tuberculosis by the gene chip is precise, fast and highly-efficient.
