Metallothionein inhibits rat vascular fibroblasts activation induced by homocysteine
- VernacularTitle:金属硫蛋白抑制同型半胱氨酸诱导的大鼠血管成纤维细胞激活
- Author:
Dongyan WANG
;
Jie GUO
;
Xia LI
;
Yang LI
;
Chaoshu TANG
;
Juxian LI
- Publication Type:Journal Article
- Keywords:
Metallothionein;
Homocysteine;
Fibroblasts;
Rats
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of exogenous metallothionein (MT) and ZnCl 2-induced MT production on biological action of homocysteine(HCY)in vascular fibroblasts METHODS: -TdR, -Pro incorporation and LDH leakage were measured, the cellular viabilities were calculated by trypan blue exclusion test and the intracellular contents of MT were assayed by [ 109 Cd]-hemoglobin saturation method in cultured rat vascular fibroblasts. RESULTS: Proliferation, collagen production of vascular fibroblasts in HCY-treated group were significantly increased compared with control group in a concentration-depedant manner. HCY (500 ?mol/L) increased LDH leakage and decreased the cellular viabilities ( P
