Influence of the serum of Radix Ophiopogonis on the apoptosis related gene expression and intracellular Ca~(2+) in vascular endothelial cells treated with LPS
- VernacularTitle:麦冬药物血清对血管内皮细胞凋亡相关基因表达及胞内Ca~(2+)的影响
- Author:
Xu ZHANG
;
Chaoying ZHANG
;
Wen WANG
;
Dongqing XU
;
Ji YANG
- Publication Type:Journal Article
- Keywords:
Radix Ophiopogonis;
Vascular endothelium;
Calcium;
Genes, bcl-2
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the molecular mechanism of Radix Ophioponis against vascular endothelial cell (VEC) apoptosis induced by LPS. METHODS: The apoptosis of human umbilical vascular endothelial cells(HUVEC) was induced by lipopolysaccharide(LPS). The influence of Radix Ophiopogonis on the expression of bcl-2 and intracellular Ca 2+ was detected by flow cytometry and laser confocal microscope. RESULTS: The serum containing Radix Ophiopogonis suppressed the increase in bcl-2 expression and overloading of Ca 2+ induced by LPS in HUVEC.CONCLUSION: The mechanism of Radix Ophiopogonis against HUVEC apotosis may be related with its regulatory effect on bcl-2 expression and remission of Ca 2+ overloading.
