Inhibitory effect of different target-side antisense oligonucleotides on bFGF expression in SWO-38 cells
- VernacularTitle:不同作用靶点的bFGF反义脱氧寡核苷酸对胶质瘤SWO-38细胞效应的观察
- Author:
Yanfang QIN
;
Xueyun ZHONG
;
Yunxia CHEN
- Publication Type:Journal Article
- Keywords:
Fibroblast growth factor 2;
Oligonucleotides,antisense;
Glioma
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: Three different antisense oligonucleotides complementary to basic fibroblast growth factor (bFGF) mRNA were compared in inhibitory effect on gene targeted expression. METHODS: After transfecting bFGF antisense oligonucleotides (asODN) into SWO-38 cells by lipofectin,the proliferation of cells was identified by MTT method, apoptosis was examined by flow cytometric cell cycle analysis and the expression levers of bFGF were detected by Western-blotting. RESULTS: There were 49%,33%,51% inhibition of cell growth and 35%, 27%, 18% cell apoptosis after asODN1, asODN2 and asODN3 treatment.In addition,the decrease in bFGF protein was 63%, 42%, 11%, respectively. CONCLUSION: The data suggeste that asODN1 is a potent target to bFGF mRNA, which inhibits cell growth and induces apoptosis in SWO-38 cells.
