Induction of apoptosis in mouse fibroblast cell line L929 by arachidonic acid
- VernacularTitle:高浓度花生四烯酸诱导小鼠成纤维细胞L929凋亡的研究
- Author:
Yun WANG
;
Binghua WANG
;
Zhaoming DAI
;
Jialing HONG
;
Lida CHEN
;
Weibin CAI
- Publication Type:Journal Article
- Keywords:
Arachidonic acids;
Fibroblasts;
Apoptosis;
Lipid peroxidation
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe whether arachidonic acid (AA) could induce apoptosis in mouse fibroblast cell line L929 and the potential mechanism involved. METHODS: The viability and damaged degree of L929 was monitored by MTT and the release of lactate dehydrogenase (LDH). Lipid peroxidation in L929 was measured as malondialdehyde (MDA) content by colorimetric assay. Hoechst 33258 staining was used to observe AA-induced morphological changes. Agarose gel electrophoresis was used to detect DNA fragmentation. RESULTS: Treatment of L929 cell with AA for 24 h, in the range of 40-160 ?mol/L, caused a great decrease in cell survival and increased MDA contents and the release of LDH simultaneously( P
