Insulin induced LPL mRNA expression in THP-1 macrophage and acceleration of transferring the macrophage to foam cell
- VernacularTitle:胰岛素对THP-1细胞脂蛋白脂酶mRNA表达及细胞泡沫化的影响
- Author:
Xiaogang ZHANG
;
Yunzhen CHEN
;
Han LEI
;
Zhoubi WANG
- Publication Type:Journal Article
- Keywords:
Insulin;
Foam cells;
Lipoprotein lipase
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of insulin on ox-LDL transferring the THP-1 cells to foam cells and influencing the LPL mRNA expression in THP-1 cells. METHODS: THP-1 cells were incubated with 50 mg/L ox-LDL and insulin at concentrations of 10 mU/L,100 mU/L,1 000 mU/L and 10 000 mU/L, respectively. The expression of LPL mRNA in cells was detected by RT-PCR. Lipoprotein lipase of THP-1 cells was presented by no-specific lipase staining. THP-1 cells were stained with oil red O. Accumulation of total cholesterol (TC) in THP-1 cells was determined with oxidase assay. RESULTS: In 100 mU/L?1 000 mU/L?10 000 mU/L insulin groups, LPL mRNA expression increased 2 times, the average cell perilength was longer, the percentage of positive oil red O staining cells was significant higher, the content of cholesterol in THP-1 cells was higher than in ox-LDL control ( P
