Changes in terminal sialyl residues linked to N-glycans in the tissue of bile duct carcinoma and bile glycoproteins
- VernacularTitle:胆管癌组织及其胆汁糖蛋白末端唾液酸结构的改变及其意义
- Author:
Xiaoling JIN
;
Shusen ZHENG
;
Bingsheng WANG
;
Huili CHEN
- Publication Type:Journal Article
- Keywords:
Bile duct neoplasms;
Glycoproteins;
Sialic acids;
Agglutinins
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the clinical significance and characteristics about terminal sialyl residues linked to N-glycans in the tissue and bile glycoproteins from bile duct carcinoma. MethodsThe mRNA expression of sialyltransferases, including ST3Gal-Ⅲ and ST6Gal-Ⅰ, was detected in the tissue extracts of 35 cases with bile duct carcinoma(BDC) and 35 cases of benign biliary disease (BBD) by semiquantitative reverse transcription-polymerase chain reaction(RT-PCR). With the lectin of WGA(Triticum valgaris agglutinin), which can recognise the terminal sialyl residues in N-glycans, all the specimens of BDC and BBD were subject to lectin histochemical staining, followed by computer-aided digital analysis. Lectin dot-blotting with horseradish peroxidase-labeled WGA was performed for the bile samples from each of the 35 cases of BDC and BBD. ResultsThe mRNA levels of ST3Gal-Ⅲ and ST6Gal-Ⅰ elevated in BDC(P
