The expression and clinical significance of substance P high affinity receptor-neurokinin-1 receptor in Crohn′s disease
- VernacularTitle:P物质受体NK-1R在克罗恩病组织中的表达和临床意义
- Author:
Xin SHI
;
Nairong GAO
;
Qingming GUO
;
Mingdong HUO
;
Haolin HU
;
Wenhao TANG
;
Helmut FRIESS
- Publication Type:Journal Article
- Keywords:
Crohn′s disease;
Receptors, neurokinin-1
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of substance P (SP) and its neurokinin-1 receptor (NK-1R) in the pathophysiologic process of Crohn′s disease. Methods In 23 surgical patients of Crohn′s disease and 24 healthy controls, reverse transcription polymerase chain reaction (RT-PCR) was used to determine the mRNA expression of NK-1R, Western blot analysis was used to determine NK-1R protein expression levels, and immunohistochemistry was used to localize expression of NK-1R. Results Compared with normal gut NK-1R mRNA and NK-1R protein in Crohn′s disease were overexpressed. In Crohn′s disease moderate to strong intestinal NK-1R immunoreactivity was found in the lamina propria mononuclear cells, lymphoid follicles, and the surface and crypt epithelium, lymphoid follicles, submucosal vessels, smooth muscle and myoenteric plexus neurones. Conclusions In cases of Crohn′s disease, overexpression of NK-1R may disturb neuropeptides loop balance, and may be involved in the pathophysiological change in this disease.
