Determination of Endotoxin in Injectio Puerarin by Kinetic Turbimetric Assay
- VernacularTitle:动态浊度法定量检测葛根素注射液中细菌内毒素
- Author:
Ji WU
;
Yan SUN
;
Jing LIU
;
Yunchuan WANG
- Publication Type:Journal Article
- Keywords:
injectio puerarin;
bacterial endotoxin;
kinetic turbimetric assay;
limulus test agent
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To approach the feasibility of replacing pyrogen test by bacterial endotoxin test(BET)in assaying the injectio puerarin.METHODS:Kinetic turbimetric assay(KTA)was used to determine the bacterial endotoxin,and an inhi?bition and strengthen test by using the EDS-99bacterial endotoxin systems was adopted for assaying injectio puerarin.RES_ ULTS:The regression equation of standard curve was logT=2.7957—0.2422logC,r=—0.9973;Injectio puerarin did not interfere with limulus test agent in1∶60dilution.CONCLUSION:This method is slight in interference and accurate in de?tection result,and can be used in routine assay.
