Determination of Content of Baicalin in Kugan Granules With HPLC
- VernacularTitle:高效液相色谱法测定苦甘颗粒中黄芩苷的含量
- Author:
Shanxue JIA
;
Wei ZHAO
;
Junhua ZHANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Kugan granules;
baicalin;
determination of content
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a HPLC method for determination of the content of baicalin in Kugan gran?ules.METHODS:ODS-C 18 column was used and methanol-water-acetic acid(45∶55∶1)was used as mobile phase and UV detection wavelength was274nm.RESULTS:There was a good linear relationship between the concentration of Baicalin and absorption area value in range of0.25?g～2.5?g r=0.9992.The average recovery was98.4%with RSD=1.09%(n=5). CONCLUSION:The method was highly repeatable and accurate.
