Preliminary Studies on Hepatic Tissue Proteome of HBV Relative Hepatocellular Carcinoma by Two-Dimensional Gel Electrophoresis
- VernacularTitle:应用双向电泳对HBV相关性肝细胞癌肝组织蛋白质组的初步研究
- Author:
Yunzhu LONG
;
Xuegong FAN
;
Ning LI
- Publication Type:Journal Article
- Keywords:
2-DE;
HBV infection;
Hepatocellular carcinoma;
Hepatic tissue;
Proteome
- From:
Journal of Chinese Physician
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study was designed to analyze the proteome differences between cancer tissue and surrounding-cancer tissue using Two-Dimensional Gel Electrophoresis (2-DE) in patients with HBV relative HCC.Methods Immobile phase pH gradients (IPGs) for isoelectric focusing of proteins were used as the first dimension,and SDS-polyacrglamide gel electrophoresis(PAGE) as the second dimension. The gels were stained by silver, scanned by ImageScanner, analyzed with ImageMast software.Results Average spots expressed in cancer tissue,cirrhosis tissue and chronic hepatitis tissue were significantly different(P
