Correlation Between Apolipoprotein E Gene Polymorphism and Alzheimer and Coronary Heart Disease
- VernacularTitle:载脂蛋白E基因多态性与老年性痴呆、老年冠心病关系的研究
- Author:
Lichun CAO
;
Huaxin LIU
;
Guilian FU
- Publication Type:Journal Article
- Keywords:
Apolipoprotein E Polymorphism;
Alzheimer;
Coronary heart disease
- From:
Journal of Chinese Physician
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Apolipoprotein E(ApoE) gene polymorphism and alzheimer and coronary heart disease was studied ,and the correlation between them was evaluated.Methods The polymerase chain reaction-restriction fragment length polymorphism was used to amply 227 base-pari DNA fragment in the fourth exon of ApoE gene,and digested them with restriction enzyme CfoI,electrophoresis on 8% polyacrylamaid gel for the cleavaged products,subsequently genotype was determined by silver staining and allele frequencies were counted.Results apoE genotype and allele frequencies was detected in 33 case with Alzheimer and 37 case with coronary heart disease.The results showed that the frequencies of ApoE ? 4 was significantly higher than that in age-matched controls(P
