Study on Effect on TCR/CD_3 Complex-mediated [Ca~(++)]i Responses in T Cells From Patients With SLE by Thapsigargin and EGTA
- VernacularTitle:Thapsigargin及EGTA对SLE T细胞TCR/CD_3复合物介导的[Ca~(2+)]i反应的影响
- Author:
Qinyong YANG
;
Yuanxing LIAO
;
Zongfa WANG
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus;
Systemic;
T cells;
TCR/CD 3;
Signal transduction;
Ca 2+
- From:
Journal of Chinese Physician
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To demonstrate that SLE T cells primary function disordor was related to abnormal [Ca 2+ ]i responses,and to investigate the reason of abnormal [Ca 2+ ]i responses.Methods After cross-linking of anti-CD 3 mAb to sheep anti-mouse IgG and stimulating T cells,the changes of free calciumion [Ca 2+ ]i within T cells under interference of Thapsigarain and EGTA was observed respectively successively for 10 minutes by an adhesion cytometry.The relation between [Ca 2+ ]i responses in SLE T cells and InsP 3 levels was evaluated .Results The basic [Ca 2+ ]i response in T cells from SLE patients was similar to that from normal control(P=0 105),peak and plateau [Ca 2+ ]i responses were significantly higher in the group of SLE patients(P
