Presentation of an Endogenous MHC ClassⅠRestricted Autoantigen by Keratinocytes in Vitro
- VernacularTitle:角质形成细胞对MHC-Ⅰ限制的自身抗原呈递作用的体外研究
- Author:
Xinghua GAO
;
Kosaka HIROSHI
;
Haiyan XU
;
Yuming ZHAO
;
Hongduo CHEN
;
Yoshikawa KUNIHIKU
- Publication Type:Journal Article
- Keywords:
Keratinocytes;
Antigen presentation;
CD8-positive T-lymphocytes
- From:
Chinese Journal of Dermatology
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the antigen presenting function of keratinocytes to CD8 + T cells with specific TCR.Methods PAM212transfectants,which express H-2K b /ovalbumin complex,aa257-264,were used as stimulating cells,and naive or primed CD8 + T cells with ovalbumin specific TCR were used as respon-ders.3 H-thymidin uptake values were used as indicators of T cell proliferation.Results With prestimulation of IFN-?,the proliferation of primed T cells but not naive T cells could be stimulated by PAM-OK cells.Conclusion Keratinocytes have the potential to stimulate the proliferation of TCR specific CD8 + T cells,which might explain the persistence of local immune reaction in some involved skin.
