Effects of IL-6 and IL-1? on the peripheral blood polymorphonuclear neutrophil apoptosis postburn in rats
- VernacularTitle:烫伤后IL-6和IL-1?对大鼠外周血中性粒细胞凋亡的影响
- Author:
Jiang ZHENG
;
Peiyuan XIA
;
Xiaojian QIN
;
Liping GAO
;
Guangxi XIAO
- Publication Type:Journal Article
- Keywords:
Burns;
Neutrophils;
Apoptosis;
Cytokines;
Caspases
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of IL-6 and IL-1? on the blood polymorphonuclear-neutrophils(PMN) apoptosis postburn. METHODS: Wistar rats inflicted by 30% total body surface area (TBSA) Ⅲ degree scalding were employed as the model. PMN were isolated by density gradient centrifugation using Percoll-hypaque and labeled with TdT-mediated and dUTP nick end labeling (TUNEL) and analyzed by flow cytometric analysis. The intracellular caspase-3 activation and the serum levels of IL-6 and IL-1? were analyzed by fluorometric immunosorbent enzyme assay and enzyme-linked immunosorbent assay, respectively. RESULTS: The serum IL-6 levels (?g/L) in groups of 3, 6, 12, 24 and 48 h postburn (9 14?1 16, 12 49?1 14, 3 01?0 75, 1 41?0 28 and 1 56?0 43 in turn) and IL-1? (ng/L) in groups of 3, 6, 12 h postburn (90 08?8 39, 320 93?14 48 and 47 84?5 19) were much higher than IL-6 (0 24?0 07) and IL-1? (27 65?4 86) in control group ( P
