Dendritic cells pulsed with antigen MAGE-3 induce antitumor immune response
- VernacularTitle:人黑色素瘤抗原-3冲击的树突状细胞诱导抗肿瘤免疫应答
- Author:
Xiaodong SUN
;
Xinge LIU
;
Jinmi WU
- Publication Type:Journal Article
- Keywords:
Melanoma;
Dendritic cells;
T-lymphocytes, cytotoxic;
Immune response
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate antitumor immune response induced by Dendritic cells (DCs) pulsed with antigen MAGE-3. METHODS: DCs were obtained from peripheral blood mononuclear cells induced with granulocyte/macrophage colony-stimulating factor (GM-CSF), interleukin-4 (IL-4), tumor necrosis factor-?(TNF-?), and pulsed with antigen MAGE-3. After cocultured with DCs for 7 days, T cells were collected and special CTLs were studied. RESULTS: T cells cocultured with MAGE-3 antigen pulsed DCs effectively killed the MAGE-3 positive MKN-45 tumor cells in vitro . CONCLUSION: DCs pulsed with MAGE-3 are able to present antigen effectively, activate antigen-specific CTLs and induce special antitumor immune response in vitro.
