The consensus oligonucleotide competitive binding with NF-?B inhibits TNF production
- VernacularTitle:共有序列寡核苷酸竞争结合NF-?B以减少TNF产生的实验研究
- Author:
Ning CHEN
;
Jing YE
;
Te TANG
;
Xueliang ZHU
- Publication Type:Journal Article
- Keywords:
Endotoxins;
Oligonucleotides;
NF-?B;
Tumor necrosis factor;
Multiple organ failure
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate whether a consensus oligonucleotide, which contains nuclear factor-kappa B(NF-?B) binding site, decreases TNF production stimulated by lipopolysaccharide(LPS). METHODS: The thiophosphoric acid modified oligonucleotides were transferred into rat peritoneal macrophages directly, TNF content in LPS-stimulated cell culture supernatant was determined by ELISA.RESULTS: Adding the consensus oligonucleotides with NF-?B binding site into macrophages markedly decreased TNF production following LPS stimulation. CONCLUSION: These results show that use of the consensus oligonucleotides with NF-?B binding site, which can combine with NF-?B, can block or decrease TNF production induced by LPS in macrophages.
