The construction of dicistron system to improve the expression level of bFGF in Escherichia coli
- VernacularTitle:利用双顺反子系统在大肠杆菌中高表达bFGF(英文)
- Author:
Gengsheng HUANG
;
Ju WANG
;
Fenyong SUN
;
Zhiying LI
;
Ling ZHANG
;
An HONG
;
Guishen LI
- Publication Type:Journal Article
- Keywords:
Dicistron;
Fibroblast growth factor 2;
Escherichia coli
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the condition necessary for high-level expression of basic fibroblast growth factor (hbFGF) in Escherichia coli, a dicistron system was constructed. METHODS: The phage T7 gene ?10 N-terminal sequence, present in the expression plasmid pET-3c, was incorporated into the first cistron, the coding region for bFGF was incorporated into the second, and both were under the control of a transcription promotor recognized by the bacteriophage T7 RNA polymerase. RESULTS: Recombinants were transformed into BL21(DE3) and induced by IPTG for expression, accumulation of up to 20% of the total cell protein of bFGF were obtained, showing bioactivity indistinguishable from standard protein. CONCLUSION: It suggested that such dicistron system was effective for enhancing hbFGF expression.
