Delay of neutrophil apoptosis in lung of rats with acute lung injury
- VernacularTitle:急性肺损伤大鼠肺泡内中性粒细胞凋亡的延迟
- Author:
Zhenqian LIU
;
Senyang YU
;
Yan LIU
- Publication Type:Journal Article
- Keywords:
Neutrophils;
Apoptosis;
Interleukin-1;
Acute lung injury;
Bronchoalveolar lavage fluid
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate neutrophil(PMN) apoptosis in bronchoalveolar lavage fluid(BALF) in the course of acute lung injury(ALI). METHODS: A rat model of ALI was established by the "two-hit" method,acid instillation and subsequent lipopolysaccharide challenge. At different time,PMN from the BALF and normal peripheral blood were incubated in BALF from normal rats or rats with ALI.PMN apoptosis was determined by flow cytometric analysis of annexin V binding, and interleukin-1?(IL-1?) mRNA was measured in PMN by PCR. RESULTS: Incubated with BALF from ALI ,PMN apoptosis was delayed with elevated expression of IL-1?, and there was a significant negative correlation between the percentage of apoptotic PMN and IL-1? level. CONCLUSION: Neutrophil apoptosis is delayed during ALI, which might have relevance to elevated expression of IL-1?.
