Antisense drug targeting with VEGF mRNA designed by computer aid and inhibitory effects on K562 cell line in vitro
- VernacularTitle:血管内皮生长因子基因反义核酸的设计及其对K562细胞增殖的影响
- Author:
Jia FEI
;
Huan ZHANG
- Publication Type:Journal Article
- Keywords:
Vascular endothelial growth factors;
Leukemia;
Oligonucleotides,antisense;
K562 cells
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The effective antisense sequences targeted VEGF mRNA with computer software would be screened and designed, and effect of them on growth K562 cells and protein expression of VEGF were studied with experiments. METHODS: Seven antisense sequences were selected and synthesized, which consisted of 18-20 deoxynucleotide acid and were modified with phosphorothioate, according to principle of low free energy of overall △G 37 Overall. Cell growth was assayed by trypan blue dye exclusion assay and level of VEGF protien in the media was determined by ELISA. RESULTS: Six of seven sequences were capable of inhibing growth of K562 cells and downregulating the VEGF protein expression significantly, compared with Scrambed control group. It was found that there was a close correlation between low level of overall △G 37 and antisense effectiveness ( r =0 887, P
