Effect of p38 protein kinase on the activation of rat alveolar macrophages by lipopolysaccharide
- VernacularTitle:p38蛋白激酶对大鼠肺泡巨噬细胞活化机制的调控
- Author:
Cuiping HUANG
;
Zhenxiang ZHANG
;
Yongjian XU
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharides;
Macrophages, alveolar;
Mitogen-activated protein kinases;
Tumour necrosis factor;
Interleukin-8
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of p38 protein kinase in the activation of rat alveolar macrophages(AMs) induced by lipopolysaccharide(LPS). METHODS: Nuclear protein was extracted, p38 protein kinase in nuclear extracts was analyzed by Western blot. The concentrations of TNF-? and IL-8 in supernatant were measured by radioimmunoassay. RESULTS: The concentrations of TNF-?, IL-8 in supernatant and p38 protein kinase in nuclear extracts were increased significantly induced by LPS and blocked by SB203580, a selective inhibitor of p38 protein kinase. CONCLUSION: The inductoin of TNF-? and IL-8 in alveolar macrophages by LPS may be mediated through the activation of p38 protein kinase.