Effect of vitamin E on ox-LDL-induced monocyte/endothelium adhesion
- VernacularTitle:维生素E对氧化低密度脂蛋白诱导的单核细胞/内皮细胞粘附的干预作用
- Author:
Rongliang XU
;
Xianxian ZHAO
;
Yongwen QIN
- Publication Type:Journal Article
- Keywords:
Lipoproteins,LDL;
Endothelium;
Monocytes;
Vascular cell adhesion molecule-1;
Vitamin E;
Rabbits
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the role of ox-LDL in rabbit endothelium/circulating monocyte adhesion in vitro and the effect of vitamin E. METHODS: Cultured rabbit's endothelial cells were incubated with different concentrations of ox-LDL, then incubated with rabbit's monocytes to observe cell's adhesion. With Northern blotting, endothelial VCAM-1 mRNA expression was measured. By using vitamin E incubation before ox-LDL, above steps were repeated to observe the effect of vitamin E. RESULTS: When ox-LDL concentrations were 2 5 mg/L, 5 mg/L, 10 mg/L, monocytes adhesive to endothelium per microscope field were 132 8?20 2, 350 0?37 2, 502 6?78 8, respectively They were all significantly higher than that of control group (51 2?7 7, P
