Ginsenoside Rh_2 enhances the apoptotic effect of cisplatin on PC-3M prostatic cancer cells
- VernacularTitle:人参单体皂甙Rh_2增强顺铂对PC-3M致凋亡效应的研究
- Author:
Yan MENG
;
Guihua ZHOU
;
Yang LI
;
Xuejian ZHAO
;
Yanping CHEN
;
Xingyua MA
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Ginsenoside Rh 2;
Cisplatin;
Prostatic neoplasms
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the inducing apoptotic effect of Cisplatin (DDP) combined with Ginsenoside Rh 2 on PC-3M prostatic cancer cells in vitro. METHODS: Cell viability was examined by MTT test and the apoptosis of PC-3M by electrophoresis of NP-40 extracting fragmental DNA and flowcytometry. RESULTS: Pc-3M cells were exposed to 15.0 mg/L Rh 2 combined with 0.4 mg/L DDP: ①Cell viabililty inhibitory rate was increased by 1.44 times from 25.0% (with 0.4 mg/L DDP) to 61.03%. ②Characteristic apoptotic DNA laddering was found more obviously than using either agent alone. ③Apoptotic rate of PC-3M was increased from 0.32% (with 0.4 mg/L DDP) to 52.2%, which showed similar effect with 4.0 mg/L DDP, it was much higher than that of 15.0 mg/L Rh 2 alone (13.6%) ( P
