Determination of the Concentrations of Salicylic Acid in Serum With RP-HPLC and Its Application
- VernacularTitle:反相高效液相色谱法测定水杨酸血药浓度及其应用
- Author:
Li ZHANG
;
Lin HE
;
Jiying YU
;
Zhengzhong WU
;
Shiming SUN
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
salicylic acid;
serum concentration of drug;
pharmacokinetics
- From:
China Pharmacy
1991;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a RP-HPLC method for the determination of salicylic acid in serum and to apply this method to the pharmacokinetic and bioavailability study of salycylic acid in compound aspirin preparations.METHODS:Waters2690HPLC instrument was used with Diamonsil C 18 column(5?m,250mm?4.6mm)as stationary phase and methanol-ace?tonitrile-0.2%phosphoric acid(18∶32∶50)as mobile phase at a flow rate of1.0ml/min,and the detective wavelength was237nm.RESULTS:Calibration curve of salicylic acid was linear in the range of0.40～101.00?g/ml(r=0.9999).CONCLUS_ ION:The method is simple,sensitive,rapid and suitable for pharmacokinetic and bioavailability study of salicylic acid.
