Specific T-cells induced by human dendritic cells pulsed with tumor cell lysate against pancreatic carcinoma cells in vitro
- VernacularTitle:癌细胞裂解物修饰的DC疫苗体外诱导T细胞特异性抗胰癌免疫
- Author:
Zhaohui TANG
;
Shengquan ZOU
;
Wenhong QIU
;
Xiangping YANG
;
Fazh QIU
- Publication Type:Journal Article
- Keywords:
PANCREATIC NEOPLASMS/immunol;
DENDRTIC CELLS;
T-LYMPHOCYTES RESPONSE;
TUMOR CELL LYSATE
- From:
Chinese Journal of General Surgery
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of a specific T-cell induced by lysate-pulsed human dendrtic cells against pancreatic cancer(PC) cells . Methods Dendritic cells were amplified and purified from peripheral blood of PC patient and were pulsed with tumor cells.Then they were co-cultured with autologus T-cell in vitro and divided into lysate-pulsed DCs group, unplused DCs group, lysates group,and control group. The supernatants of every co-culture group were collected and the concentration of IL-12 and IFN-? were measured by ELISA. The capacity of DC induced proliferation of T-cells was tested.The specific cytolytic activity of CTL was assessed in a 4-hr 51 Cr-release assay. Results Difference in the concentration of IL-12 and IFN-? was statistically significant in lysate-pulsed DCs group(1161?239 pg/ml and 1044?312 pg/ml)than these in unplused DCs group and lysates group (P
