Separation of SO-Rb_ (50)cells antigen corresponding to the monoclonal antibody of anti-retinoblastoma
- VernacularTitle:抗视网膜母细胞瘤细胞株SO-Rb_(50)单克隆抗体相应抗原的提取
- Author:
Fangwei YING
;
Yongping LI
;
Song TANG
- Publication Type:Journal Article
- Keywords:
Retinoblastoma;
Antibodies, monoclonal;
Antigens/isolalion & purification
- From:
Chinese Journal of Ocular Fundus Diseases
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To separate the SO-Rb 50cells antigen corresponding to the monoclonal antibody of anti-retinoblastoma. Methods The antigen corresponding to the monoclonal antibody of anti-retinoblastoma was separated elementarily by ion-exchange chromatography, and was identified by dot-blotting using the monoclonal antibody of anti-retinoblastoma. The target protein band of the antigen was separated in light of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Results A special unmixed band of SO-Rb 50cells antigen was separated with the relative molecular weight of 83?103. Conclusion The antigen corresponding to the monoclonal antibody of anti-retinoblastoma could be separated from SO-Rb 50cells.
