The inhibitive effect of adenovirus mediated CD gene and 5-FC on proliferative human retinal pigment epithelialcells
- VernacularTitle:腺病毒转导的胞嘧啶脱氨酶基因联合5-氟胞嘧啶对人视网膜色素上皮细胞增殖的抑制作用
- Author:
Wenying WANG
;
Mi YAN
;
Junjun ZHANG
- Publication Type:Journal Article
- Keywords:
Human retinal pigment epithelial cells;
Cytosine deaminase gene;
5-fluorocytosine;
5-fluorouracil
- From:
Chinese Journal of Ocular Fundus Diseases
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitive effect of adenovirus mediated CD gene and 5-FC on proliferative human retinal pigment epithelial (HRPE) cells, and to search for an effective method to take precautions against proliferative vitroretinopathy (PVR). Method Different concentrations of CD and 5-FC were added respectively to the cultured third-growth-generation HRPE cells.Transferance rate was detected by positive HRPE cells marked by X-gal and LacZ. The number of HRPE cells were counted and evaluated by methylthiazol-tetrazollium (MTT) method. Results The adenovirus mediated CD gene could be transfered into HRPE cells with a dose-dependent manner. Positive HRPE cells with CD gene could transform 5-FC to 5-Fu,which could inhibit the increase of HRPE cells effectively. No obvious bystander effect on the growth of HRPE cells was detected. Conclusions The adenovirus may introduce a foreign gene into cultured HRPE cells efficiently. It could be a good method to treat and prevent PVR by medication.
