Cloning and Identifying the Differential Genes in Resistant Neisseria Gonorrhoeae
- VernacularTitle:耐药淋球菌差异DNA消减文库的构建及初步筛选
- Author:
Mingchun JI
;
Hua YAN
;
Hongju CHEN
;
Houfeng SHEN
- Publication Type:Journal Article
- Keywords:
Neisseria gonorrhoeae;
Drug resistance,bacterial;
Suppression subtractive hybridiza-tion;
Sequence analysis,DNA;
Gene library
- From:
Chinese Journal of Dermatology
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone and study the antimicrobial-resistant gene in Neisseria gonorrhoeae.Methods The gene library,which contains the differential genes of antimicrobial resistant strains and stan-dard reference strains of Neisseria gonorrhoeae,was constructed using a technique known as suppression subtractive hybridization(SSH).Then the antimicrobial resistance associated genes were cloned and ana-lyzed.Results Subtractive gene library in antimicrobial-resistant Neisseria gonorrhoeae was successfully constructed,which contains2500positive clones.Sequence analysis was performed on5clones.The se-quences of these five clones were unknown previously.Conclusions The subtractive DNA library is succes-sively constructed which may provide an important clue for studying the mechanism of antimicrobial resis-tance in Neisseria gonorrhoeae.
