The inhibitory effect of quercetin on in vitro activation of T lymphocytes
- VernacularTitle:槲皮素对小鼠T细胞体外活化的抑制作用
- Author:
Nan WANG
;
Yaoying ZENG
;
Xianhui HE
;
Jingxian ZHAO
;
Haixia QIU
- Publication Type:Journal Article
- Keywords:
Quercetin;
CD69;
T-lymphocytes;
Flow cytometry
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the inhibitory effect of quercetin on in vitro activation of T lymphocytes by polyclonal activators with CD69 expression as an activation marker. METHODS: After being separated from lymphoid nodes of a C57BL/6 mouse, the lymphocytes were exposed to polyclonal activators (PDB or Con A) with or without quercetin. Then they were harvested at 2 h, 6 h and 24 h, respectively. The expressional rates of CD69 on T lymphocytes were assessed by two-color immunofluorescent staining and flow cytometry, and the inhibitory rates of quercetin at different time points were estimated. RESULTS: Quercetin had no effect on the expressional rate of CD69 on T lymphocytes under resting states. After the stimulation with PDB or Con A, the expressional rates of CD69 on T lymphocytes in the present of quercetin (10 ?mol/L) showed significant decrease compared with those of control groups at different time points (P
