Effects of PKC-? asODN and PKA-ⅠasODN on growth andproliferation of the CNE-2Z cells of nasopharyngeal carcinoma
- VernacularTitle:PKC-?、PKA-Ⅰ反义核酸对鼻咽癌CNE-2Z细胞生长增殖的影响
- Author:
Baohua ZHU
;
Peichun HUANG
;
Bo BAO
;
Jin CHEN
- Publication Type:Journal Article
- Keywords:
Oligonucleotides, antisense;
Nasopharyngeal neoplasms;
Protein kinase C
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of antisense oligonucleotides (asODN) of PKC-? and PKA-Ⅰon growth and proliferation of the CNE-2Z cells. METHODS: The expression of PKC-? and PKA-Ⅰ was observed with immunohistochemistry method. The asODNs of (1)PKC-?, (2)PKA-Ⅰ, (3)PKC-? and PKA-Ⅰ, were transfected into CNE-2Z cells by lipofectin (LP), and a random sequence as a control was used. The cell growth index (GI) and the clone formation rate of CNE-2Z were detected by MTT colorimetric assay and soft agar assy, respectively. RESULTS: The expression of PKC-? or PKA-Ⅰin CNE-2Z in experimental group were both significantly lower than that of control group(P
