Effect of desflurane pretreatment on the density of muscarinic receptors on hypoxic/reoxygenated cardiac myocytes
- VernacularTitle:地氟醚预处理对缺氧/复氧大鼠心肌细胞M受体密度的影响
- Author:
Yuewu YANG
;
Yannan HANG
;
Dajin SUN
- Publication Type:Journal Article
- Keywords:
Myocytes,cardiac;
Receptors,muscarinic;
Ischeraic preconditioning,myocardial;
Desflurane
- From:
Chinese Journal of Anesthesiology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of desflurane pretreatment on the density of muscarinic (M)-receptors on anoxic/reoxygenated rat cardiac myocytes. Methods Myocytes were obtained from ventricle of 2-3 day SD rats and incubated for 3 days. Then they were randomly allocated to one of 5 groups: group I received no treatment and served as control ( n = 5); group Ⅱ underwent 2 h hypoxia (liquid culture medium was insufflated with a gas mixture of 1 % CO2 , 1 % O2 and 98% N2) followed by 1h reoxygenation ( n = 6) ; group Ⅲ was pre-treated with 1.5 MAC desflurane (9% ) for 20 min, followed by 10 min washout before the same duration of hypoxia/reoxygenation as in group II ( n = 6) ; group IV underwent longer duration of hypoxia (48 h) followed by 24 reoxygenation ( n = 5) ; group V was pre-treated with 1.5 MAC desflurane (9% ) for 20 min followed by 10 min washout, then underwent longer duration of hypoxia/reoxygenation as in group IV ( n = 5) . The density of M-receptors was measured by the radio-ligand binding assay (RLBA) .Results The density of M-receptors was significantly lower in group Ⅱthan that in group I ( P
